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. 2011 Jul 30;33(24):3114–3123. doi: 10.1093/eurheartj/ehr245

Figure 4.

Figure 4

Phosphatidylinositol 3-kinase inhibition blunts interleukin-7 induced up-regulation of monocyte chemoattractant protein-1 and vascular cell adhesion molecule-1, but not intercellular adhesion molecule-1. Cellular RNA was extracted for qRT-PCR 4 h after incubation with interleukin-7. Cellular proteins were extracted 6 h after incubation for immunoblot analysis. (A) Effects of inhibitors on mRNA levels. *P< 0.05 (vs. interleukin-7), †P< 0.01 (vs. interleukin-7), ‡P< 0.001 (vs. control); n = 5. (B) Effects of inhibitors on monocyte chemoattractant protein-1 level. *P< 0.001 (vs. interleukin-7), †P< 0.0001 (vs. control), ‡P< 0.00001 (vs. interleukin-7). Monocyte chemoattractant protein-1 levels in control were 191 ± 10 pg/mL; n = 3. (C) Effects of inhibitors on cell adhesion molecule protein levels. (D) Phosphatidylinositol 3-kinase inhibitor blocks interleukin-7 stimulated AKT phosphorylation. (E) Inhibition of THP-1 cell adhesion to human aortic endothelial cells by LY294002. *P< 0.05 (vs. interleukin-7), †P< 0.01 (vs. control). n = 6. All experiments were repeated two to three times.