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Formation of reactive oxygen species (ROS) in polymorphonuclear (PMN) cells. Freshly isolated primary human PMN cells were either untreated or pretreated with serial dilutions of SBLS aqueous or ethanol extracts, then cultured under conditions of oxidative stress to provoke intracellular ROS formation. All test conditions were performed in quadruplicate. The data are shown as mean±SD and represent one of three separate experiments using cells from three different donors (*P<.05).
