Table 3.
Changes in Cytokine Levels After Exposure of Peripheral Blood Mononuclear Cells to SBLS Ethanol Extract
| Target | MFI (untreated)a | MFI (SBLS)b | Fold changec | Concentration (ng/mL)d | |
|---|---|---|---|---|---|
| IL-1β | 1.00 | 9.00 | 9 | <12.07 | |
| IL-1Rα | 2.50 | 21.00 | 8 | 72.58 | |
| IL-6 | 6.50 | 1129.50 | 174 | 430.68 | |
| IL-7 | 0.75 | 4.75 | 6 | 16.73 | |
| CXCL8 | IL-8 | 660.25 | 8511.50 | 13 | >388.9 |
| CSIF | IL-10 | 0.50 | 7.50 | 15 | <30.25 |
| IL-12 | 2.50 | 11.50 | 5 | 13.08 | |
| IL-13 | 0.50 | 2.50 | 5 | <8.78 | |
| IL-15 | 1.00 | 8.50 | 9 | <11.61 | |
| CCL2 | MCP1 | 13.00 | 1890.25 | 145 | >592.51 |
| CCL3 | MIP-1α | 0.50 | 120.50 | 241 | 62.97 |
| CCL4 | MIP-1β | 2.50 | 418.75 | 168 | 127.27 |
| CCL5 | RANTES | 156.00 | 337.25 | 2 | 94.84 |
| IFN-α | 1.00 | 12.00 | 12 | 33.38 | |
| CXCL10 | IP10 | 1.00 | 5.50 | 6 | <1.62 |
No change: IL-2, IL-2R, IL-4, IL-5, IL-17, eotaxin/CCL11, GM-CSF, IFN-γ, MIG/CXCL9, TNF-α.
a
Cytokine levels in untreated cell cultures.
b
SBLS ethanol extract (111 mg/L).
c
Calculated from net mean fluorescence intensity (MFI) data.
d
Calculated from the standard curves for the Luminex array. Concentrations reflect treatment with SBLS.
SBLS, Sorghum bicolor leaf sheaths.