Table 1.
Gene primer sequences and GenBank accession number of rat for the real-time PCR
| Genes | Primer Sequences | GenBank Accession Number | Product Size (bp) |
|---|---|---|---|
|
Imd |
F: 5′-GCTGATGGTCACGGTAAC-3′ |
NM_201426.1 |
122 |
|
R: 5′-CGCTGGAAGGAATCTTGG-3′ | |||
|
Crlr |
F: 5′-CCAAACAGACTTGGGAGTCACTAGG-3′ |
NM_012717.1 |
323 |
|
R: 5′- GCTGTCTTCTCTTTCTCATGCGTGC-3′ | |||
|
Ramp1 |
F: 5′- CACTCACTGCACCAAACTCGTG-3′ |
NM_031645.1 |
196 |
|
R: 5′- CAGTCATGAGCAGTGTGACCGTAA-3′ | |||
|
Ramp2 |
F: 5′- AGGTATTACAGCAACCTGCGGT-3′ |
NM_031646.1 |
163 |
|
R: 5′- ACATCCTCTGGGGGATCGGAGA-3′ | |||
|
Ramp3 |
F: 5′- ACCTGTCGGAGTTCATCGTG-3′ |
NM_020100.2 |
180 |
|
R: 5′- ACTTCATCCGGGGGGTCTTC-3′ | |||
|
Actb |
F: 5′- GGAAATCGTGCGTGACATTA-3′ |
NM_031144.2 |
183 |
| R: 5′- AGGAAGGAAGGCTGGAAGAG-3′ |