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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Photochem Photobiol. 2011 Aug 25;87(6):1321–1329. doi: 10.1111/j.1751-1097.2011.00979.x

Figure 3.

Figure 3

Photosensitization of α-crystallin by porphyrins promotes cross-linking and accumulation of NFK. Aliquots of α-crystallin were incubated without addition of porphyrin or with 1 μM and 10 μM of the indicated porphyrins and were either kept in the dark (no UVA) or were exposed to 10, 20 or 40 min of UVA irradiation as described in the materials and methods. For each porphyrin duplicate gels containing 13 μg of protein per lane were subjected to SDS-PAGE electrophoresis under reducing conditions and one gel was stained with Coomassie blue (top panels) and the other was transferred to nitrocellulose for western analysis with anti-NFK antiserum (bottom panels).