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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: J Immunol. 2008 Aug 15;181(4):2620–2625. doi: 10.4049/jimmunol.181.4.2620

Figure 2. Hybrid joint formation in wild type, Atm-deficient and NHEJ-deficient abl pre-B cells.

Figure 2

a. Southern blot analysis of pMX-INV coding and hybrid joint formation on EcoRV/NcoI digested genomic DNA from wild type (WT), Atm−/−, Artemis−/−, Scid and Ku70−/− abl pre-B cells treated with STI571 for the indicated time (hours, h). Fragments of the correct size for pMX-INV NR, CJ, HJ and CE are indicated. The position of the molecular weight markers is shown. Results are representative of at least two experiments on each of two independent cell lines. b. PCR analysis of pMX-INV hybrid joint formation in the samples from (a) treated with STI571 for 96 hours. The IL-2 gene PCR is shown as a DNA loading control. The PCRs were performed on serial 4-fold dilutions of genomic DNA.