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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Sep 6;32(11):e135–e146. doi: 10.1161/ATVBAHA.112.300255

Figure 1.

Figure 1

The expression domains of chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) during cardiac development. A and B, Using COUP-TFII/lacZ knock-in mice, COUP-TFII (blue) is first noted at the posterior cardiac crescent of E8 embryo shown in lateral (A) and ventral (B) views. C, The expression of COUP-TFII is largely localized in the sinus horn region at the 6-somite stage. Its expression is also shown in lateral plate mesoderm and somites. D, At E9.5, COUP-TFII is highly expressed in the sinus venosus and the atrium. E, High expression level of COUP-TFII (green) is detected in the atrium and is colocalized with the myocardium marker smooth muscle actin-α (SMA-α; red) at E9.5 (21 somites). F, COUP-TFII (green) begins to express in the endocardial atrioventricular cushion (AVC) toward the atrium later at E9.5. Area surrounding AVC (dotted rectangle) is magnified in the bottom panel. G, Sagittal section from E10.5 embryo shows high expression level of COUP-TFII (green) in the endocardium (arrowhead), atrial myocardium, and epicardium, whereas weak signal is detected in the myocardium underlying AVC. H, COUP-TFII (green) is detectable in the endocardium of the ventricle (arrow) as it is colocalized with the endothelial marker Tie2 (red). I, COUP-TFII (green) is not colocalized with the myocardial marker SMA-α in the ventricle (red). J, COUP-TFII is expressed in the endocardium and epicardium (arrow) of the ventricle at E15.5. K, From E13.5 to E18.5, high levels of COUP-TFII expression are observed in the atrium, whereas weak COUP-TFII expression is detected in the ventricle. A indicates atrium; Hf, head fold; iAVC, inferior atrioventricular cushion; LA, left atrium; LPM, lateral plate mesoderm; OFT, out-flow tract; RA, right atrium; sAVC, superior atrioventricular cushion; sh, sinus horn; ss, somites; SV, sinus venosus; V, ventricle.