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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Sep 6;32(11):e135–e146. doi: 10.1161/ATVBAHA.112.300255

Figure 5.

Figure 5

Loss of chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) does not affect the formation of proepicardium. A, Immunofluorescent staining for COUP-TFII (green) in wild-type and COUP-TFII−/− littermates at E9.5. Nuclei were counterstained with 4′,6-diamidine-2′-phenylindole dihydrochloride. B and C, Hematoxylin and eosin staining of sagittal sections obtained from wild-type and COUP-TFII−/− mutants at E9.5. C, Higher magnification of the regions shown in B. In both wild-type and COUP-TFII−/− mutants, a substantial number of epicardial progenitors cell cysts are formed and bud out from the proepicardium (PE). D, Immunohistochemistry for GATA4 in sagittal sections of E9.5 wild-type and COUP-TFII−/− embryos. All tissues are labeled with GATA4. E, Immunohistochemistry reveals Wilms tumor 1 expression in the PE and epicardial cells in wild-type and COUP-TFII−/− littermates at E9.5. A indicates atrium; OFT, outflow tract; V, ventricle.