Table 1.
pCoofy | parental vector | N-tag | C-tag | Host | fd primer | rev primer |
---|---|---|---|---|---|---|
pCoofy1 |
pETM14 |
His6 |
none |
E. coli |
3C |
ccdB |
pCoofy18 |
pCoofy1 |
His10 |
none |
E. coli |
3C |
ccdB |
pCoofy7 |
pETM14 |
S |
none |
E. coli |
3C |
ccdB |
pCoofy12 |
pCoofy1 |
OneStrep |
none |
E. coli |
3C |
ccdB |
pCoofy34 |
pCoofy12 |
S-OneStrep |
none |
E. coli |
3C |
ccdB |
pCoofy21 |
pCoofy12 |
His10-OneStrep |
none |
E. coli |
3C |
ccdB |
pCoofy19 |
pCoofy1 |
CBP |
none |
E. coli |
3C |
ccdB |
pCoofy2 |
pETM22 |
Trx-His6 |
none |
E. coli |
3C |
ccdB |
pCoofy38 |
pCoofy2 |
Trx-His10 |
none |
E. coli |
3C |
ccdB |
pCoofy3 |
pETM33 |
His6-GST |
none |
E. coli |
3C |
ccdB |
pCoofy8 |
pETM14 |
Halo |
none |
E. coli |
3C |
ccdB |
pCoofy35 |
pCoofy4 |
MBP |
none |
E. coli |
3C |
ccdB |
pCoofy4 |
pETM44 |
His6-MBP |
none |
E. coli |
3C |
ccdB |
pCoofy15 |
pETM14 |
NusA |
none |
E. coli |
3C |
ccdB |
pCoofy16 |
pCoofy15 |
His10-NusA |
none |
E. coli |
3C |
ccdB |
pCoofy5 |
pET28M-Sumo1 |
His6-Sumo1 |
none |
E. coli |
Sumo1 |
ccdB |
pCoofy6 |
pET28M-Sumo3 |
His6-Sumo3 |
none |
E. coli |
Sumo3 |
ccdB |
pCoofy17 |
pCoofy6 |
His10-Sumo3 |
none |
E. coli |
Sumo3 |
ccdB |
pCoofy22 |
pCoofy7 |
S |
His10 |
E. coli |
3C |
10His |
pCoofy31 |
pCoofy12 |
OneStrep |
His10 |
E. coli |
3C |
10His |
pCoofy36 |
pCoofy34 |
S-OneStrep |
His10 |
E. coli |
3C |
10His |
pCoofy24 |
pCoofy19 |
CBP |
His10 |
E. coli |
3C |
10His |
pCoofy23 |
pCoofy14 |
Trx |
His10 |
E. coli |
3C |
10His |
pCoofy25 |
pCoofy8 |
Halo |
His10 |
E. coli |
3C |
10His |
pCoofy37 |
pCoofy35 |
MBP |
His10 |
E. coli |
3C |
10His |
pCoofy26 |
pCoofy15 |
NusA |
His10 |
E. coli |
3C |
10His |
pCoofy32 |
pCoofy1 |
His6 |
OneStrep |
E. coli |
3C |
OneStrep |
pCoofy33 |
pCoofy18 |
His10 |
OneStrep |
E. coli |
3C |
OneStrep |
pCoofy11 |
pIEX1 |
His10 |
none |
Insect |
3C |
ccdB |
pCoofy27 |
pFastBac |
His6 |
none |
Insect |
3C |
ccdB |
pCoofy28 |
pFastBac |
His6-GST |
none |
Insect |
3C |
ccdB |
pCoofy29 |
pFastBac |
His6-MBP |
none |
Insect |
3C |
ccdB |
pCoofy40 | pTT5 | none | flexible | HEK293E | 3C | flexible |