Table 2.
Primer for vector amplification and complementary primer extensions for gene of interest
| LP1 forward vector primer | |
|---|---|
| 3C |
5' GGGCCCCTGGAACAGAACTTCCAG 3' |
| Sumo1 |
5' TCCACCGGTTTGTTCCTGGTAGAC 3' |
| Sumo3 |
5' TCCACCGGTCTGCTGCTGGAACAC 3' |
| N-tagless pET |
5' GGTATATCTCCTTCTCTAGAGGGGAATTGTTATCCGCTC 3' |
| N-tagless pFastBac |
5' GGAATTCCGCGCGCTTCGGACC 3' |
|
LP2 reverse vector primer |
|
| ccdB |
5' CGCCATTAACCTGATGTTCTGGGG 3' |
| 10His |
5' GAGCATCATCATCATCACCAC 3' |
| OneStrep |
5' AGCGCTTGGAGCCACCCGCAG 3' |
| S |
5' AAAGAAACCGCTGCTGCTAAATTCG 3' |
| HPC4 |
5' GAGGACCAGGTGGACCCCCGG 3' |
|
LP1 forward gene primer |
|
| 3C |
5' AAGTTCTGTTCCAGGGGCCC – GOI seq 3' |
| Sumo1 |
5' GTCTACCAGGAACAAACCGGTGGA – GOI seq 3' |
| Sumo3 |
5' GTGTTCCAGCAGCAGACCGGTGGA – GOI seq 3' |
|
LP2 reverse gene primer |
|
| ccdB |
5' CCCCAGAACATCAGGTTAATGGCG - Stop - GOI - seq 3' |
| 10His |
5' GTGGTGATGATGATGATGCTC - Stop - GOI - seq 3' |
| OneStrep |
5' CTGCGGGTGGCTCCAAGCGCT - Stop - GOI - seq 3' |
| S |
5' AGCAGCAGCGGTTTCTTT - Stop - GOI - seq 3' |
| CBP |
5' CTTCCACCGCCGCTTCATC - Stop - GOI - seq 3' |
| HPC4 |
5' GGGGTCCACCTGGTCCTC – Stop - GOI - seq 3' |
| Æ54CPD54 | 5' CAGGATCTTGCCGCTGCC – Stop - GOI - seq 3' |