Table 1. Stages of soybean seed development and RNA-Seq data.

Stage	Age/Weight	Tissue	Developmental Events	Bio Rep 1 Reads	Bio Rep 2 Reads
1	4 DAF	Whole seed	Spherical embryo; acellular endosperm	11.5 M	51.1 M
2	12–14 DAF	Whole seed	Cotyledons elongate; endosperm forms cell walls	24.3 M	183.5 M
3	22–24 DAF	Whole seed	Cotyledons have organelles; first trifoliate leaf initiates	77.6 M	181.9 M
4	5–6 mg	Whole seed	Endosperm depleted; rapid accumulation of water, nutrients	30.5 M	149.9 M
5	100–200 mg	Cotyledon	Nutrient accumulation continues; storage proteins synthesized	18.1 M	129.7 M
6	400–500 mg	Cotyledon	Peak of fresh weight; embryo prepares for desiccation	34.1 M	122.0 M
7	100–200 mg	Whole seed, dry	Quiescent embryo; low moisture content	30.2 M	64.0 M

Shown are the seven stages of soybean seed development used for RNA-Seq. Days after flowering (DAF) or fresh weight in milligrams (mg) is shown along with tissue and the number of processed reads obtained from two biological replicates of RNA high-throughput sequencing (in millions, M). Key events in development are noted.