Figure 2. Different reverse transcription setups provided by three suppliers.

(A) Random 6 mer were used in 2 and 5, oligo dT primer in 3 and 6, and both together in 1, 4 and 7 (abscissa); Cq on ordinate shows differences among the tested reverse transcriptase conditions. (B) Coefficient of variation (CV). RT suppliers indicated by roman numerals: I) BioRad, II) Takara, and III) Bioline. X-axis with different RT primers: oligo dT (oligo), random 6 mers (random), and both (both).