Table 1. Details of reference genes, primers and amplicons for 12 investigated genes.
Genesymbol | Title | Accessionnumber | ChromosomalLocalization | Forward andReverse Primer | Product(bp) | Intronspanning |
GAPDH | Glyceraldehyd-3-phosphat-Dehydrogenase | NM_002046 | 12p13.31 | 5′-CGACAGTCAGCCGCATCTT-3′ 5′-CCCCATGGTGTCTGAGCG-3′ | 63 | Yes |
RPII (POLR2A) | polymerase (RNA) II (DNA directed)polypeptide A | NM_000937 | 17p13.1 | 5′-GCACCACGTCCAATGACAT-3′ 5′-GTGCGGCTGCTTCCATAA-3′ | 267 | Yes |
TBP | TATA box binding protein | NM_003194 | 6q27 | 5′-TGCACAGGAGCCAAGAGTGAA-3′ 5′-CACATCACAGCTCCCCACCA-3′ | 132 | Yes |
PPIA | Peptidylprolyl isomerase A (cyclophilin A) | NM_021130 | 7p13 | 5′-AGACAAGGTCCCAAAGAC-3′ 5′-ACCACCCTGACACATAAA-3′ | 118 | Yes |
GUSB | Beta glucuronidase | NM_000181 | 7q21.11 | 5′-AGCCAGTTCCTCATCAATGG-3′ 5′-GGTAGTGGCTGGTACGGAAA-3′ | 160 | Yes |
HSPCB | Heat shock protein 90kDa alpha (cytosolic) | NM_007355 | 6p12 | 5′-TCTGGGTATCGGAAAGCAAGCC-3′ 5′-GTGCACTTCCTCAGGCATCTTG-3′ | 80 | Yes |
YWHAZ | Tyrosine 3-monooxygenase/ tryptophan5-monooxygenase activation protein,zeta polypetide | NM_003406 | 8q23.1 | 5′-ACTTTTGGTACATTGTGGCTTCAA-3′ 5′-CCGCCAGGACAAACCAGTAT-3′ | 94 | No |
SDHA | Succinate dehydrogenase complex, subunit A | NM_004168 | 5p15 | 5′-TGGGAACAAGAGGGCATCTG-3′ 5′-CCACCACTGCATCAAATTCATG-3′ | 86 | Yes |
RPS13 | ribosomal protein S13 | NM_001017 | 11p15.1 | 5′-CGAAAGCATCTTGAGAGGAACA-3′ 5′-TCGAGCCAAACGGTGAATC-3′ | 87 | Yes |
HPRT1 | Hypoxanthine phosphoribosyl-transferase 1 | NM_000194 | Xq26.1 | 5′-TGACACTGGCAAAACAATGCA-3′ 5′-GGTCCTTTTCACCAGCAAGCT-3′ | 94 | Yes |
18s | 18s rRNA | NT_167214.1 | ChrUn* | 5′-AGAAACGGCTACCACATCCA-3′ 5′-CACCAGACTTGCCCTCCA-3′ | 169 | N/A |
B4GALT6 | UDP-Gal:βGlcNAcβ 1,4-galactosyl-transferase,polypeptide 6 | NM_004775.3 | 18q12.1 | 5′-AGGAGGTCCCTATGGCACTAAC-3′ 5′-TCTCTACAGACAGGCCCATTAGTC-3′ | 89 | No |
Homo sapiens unplaced genomic contig, GRCh37.p5.