Figure 8. Reduced T cell proliferation by PLN cells from adult NOD mice infected as infants.

PLN cells from groups of age-matched mice, which had been mock- or RRV-infected either as infants (5 days of age) or adults (12 weeks of age), were harvested at 17 weeks of age. Equivalent numbers of cells from mice in each group were stimulated with antibodies to CD3 and CD28. T cell proliferation was measured by ³H-thymidine incorporation (counts/min). Data for 4×10⁵ cells/well are shown. A pool of cells from 4 (infected as infant) or 5 (infected as adult) mice was analysed in triplicate for each inoculum. * p<0.05.