Table 2. Functional hemostatic assays in whole-blood (impedance aggregometry (Multiplate), Thrombelastography (TEG), Functional fibrinogen) and plasma (TEG with or without addition of tPA to induce fibrinolysis) in nine healthy volunteers before, during and after induction of experimental endotoxemia by means of a 4 h LPS-infusion (0.5 ng/kg/hour).
| Endotoxemia (n = 9) | ||||||
| Units | 0 h | 4 h | 6 h | RM p-value | Tukey | |
| Multiplate | ||||||
| TRAPtest | U | 125±24 | 85±19 | 89±21 | <0.001 | a,b |
| U/platelet | 0.59±0.15 | 0.45±0.08 | 0.51±0.11 | 0.008 | a | |
| ADPtest | U | 79±15 | 57±12 | 55±10 | <0.001 | a,b |
| U/platelet | 0.37±0.10 | 0.30±0.04 | 0.32±0.06 | 0.064 | ||
| ASPItest | U | 93±16 | 73±17 | 82±13 | 0.028 | a |
| U/platelet | 0.42±0.10 | 0.37±0.07 | 0.45±0.04 | 0.117 | ||
| COLtest | U | 84±14 | 64±14 | 70±13 | <0.001 | a,b |
| U/platelet | 0.40±0.10 | 0.34±0.04 | 0.40±0.06 | 0.060 | ||
| Whole blood TEG | ||||||
| R | min | 8.6±2.4 | 5.5±1.2 | 6.7±2.0 | 0.003 | a |
| Angle | degrees | 57±6 | 62±7 | 58±10 | NS | |
| MA | mm | 54±6 | 59±5 | 58±3 | 0.084 | |
| G | dyn/cm2 | 6,010±1,232 | 7,229±1,487 | 6,928±934 | 0.050 | a |
| LY30 | % | 0.7±0.8 | 0.7±1.1 | 0.8±1.1 | NS | |
| LY60 | % | 3.5±2.6 | 3.7±2.9 | 4.3±3.7 | NS | |
| CLT | min | 136±38 | 71±16 | 151±5 | <0.001 | a,c |
| Whole blood Functional Fibrinogen | ||||||
| R | min | 5.6±1.2 | 5.2±1.3 | 5.7±1.5 | NS | |
| Angle | degrees | 29±8 | 32±9 | 32±11 | NS | |
| MA | mm | 14±2 | 12±2 | 12±3 | 0.049 | a |
| G | dyn/cm2 | 787±151 | 688±157 | 671±210 | 0.051 | |
| LY30 | % | 0±0 | 0±0 | 0±0 | NS | |
| LY60 | % | 0±0 | 0±0 | 0±0 | NS | |
| CLT | min | 120±62 | 80±18 | 237±221 | 0.067 | |
| Plasma TEG | ||||||
| R | min | 6.3±1.8 | 5.6±0.9 | 5.3±0.3 | 0.137 | |
| Angle | degrees | 45±13 | 52±8 | 56±7 | 0.013 | a,b |
| MA | mm | 19±3 | 19±3 | 20±3 | 0.151 | |
| G | dyn/cm2 | 1,162±221 | 1,172±217 | 1,250±245 | 0.149 | |
| LY30 | % | 0±0 | 0±0 | 0±0 | NS | |
| LY60 | % | 0±0 | 0±0 | 0±0 | NS | |
| CLT | min | 124±36 | 117±40 | 114±40 | NS | |
| Plasma TEG +tPA | ||||||
| R tPA | min | 6.2±2.1 | 5.6±0.8 | 5.1±0.5 | 0.178 | |
| Angle tPA | degrees | 44±14 | 53±8 | 57±6 | 0.011 | a,b |
| MA tPA | mm | 14±4 | 16±3 | 19±3 | <0.001 | a,b,c |
| G tPA | dyn/cm2 | 798±249 | 978±200 | 1,165±216 | <0.001 | a,b,c |
| LY30 tPA | % | 51.1±26.2 | 25.2±29.0 | 0.1±0.4 | <0.001 | a,b,c |
| LY60 tPA | % | 73.5±16.2 | 43.6±36.5 | 3.2±9.2 | <0.001 | a,b,c |
| CLT tPA | min | 26±14 | 68±58 | 117±40 | <0.001 | a,b,c |
Data are presented as means±SD. Data from volunteers were compared by repeated-measures analyses (RM) and Tukey post hoc tests: p<0.05 for 0 h vs. 4 ha, 0h vs. 6 hb and 4 h vs. 6 hc. P-values <0.2 are shown and in bold if p<0.05.
Different platelet agonists were applied in the Multiplate tests: TRAPtest, thrombin-receptor activating peptide; ADPtest, ADP; COLtest, collagen; ASPItest, arachidonic acid; TEG, thrombelastography; R, reaction time; Angle, α angle; MA, maximum amplitude; G, shear elastic modulus strength; CLT, clot lysis time; Ly30/60, percent lysis 30/60 min after MA;
tPA, tissue-type plasminogen activator. NS, non-significant.