Figure 6. Lymphoma cells trap TGF-β through heparan sulfate.

(A) Histograms showing TGF-β binding or TGF-β receptors (I, II or III) measured by flow cytometry on lymphoma B cell lines OCI-Ly10 and OCI-Ly19 or freshly-isolated CD19⁺ B cells from a representative lymphoma patient. (B) Histograms showing syndecans (SDC) measured by flow cytometry on lymphoma B cell lines or freshly-isolated CD19⁺ B cells from a representative lymphoma patient. (C) Representative histograms showing TGF-β binding to OCI-Ly19 cells pre-incubated with or without heparin (Hep). The binding capacity was expressed by mean fluorescent index (MFI) and summarized from multiple experiments (n = 7) in the graph on the right. (D) Representative histograms showing TGF-β binding to OCI-Ly19 cells pre-incubated with Hep or αTβRI (left) or αTβRII (right) alone or in combination. The binding capacity was expressed by MFI.