Figure 7. Effects of TGF-β-bearing lymphoma B cells on intratumoral T cell function.

(A) Representative histograms showing proliferation of CD4⁺ T cells, measured by CFSE staining, co-cultured with untreated or TGF-β pre-incubated OCI-Ly10 or OCI-Ly19 cells. Proliferative capacity was expressed by calculating the number of CFSE^dim cells. (B) Summarized data showing proliferation of CD4⁺ T cells co-cultured with untreated or TGF-β pre-incubated OCI-Ly10 (n = 5) or OCI-Ly19 (n = 8) cells. Changes in proliferation of CD4⁺ T cells were expressed by percentage of CFSE^dim cells in TGF-β pre-incubated group over untreated group. (C) Representative dot plots (n = 3) showing the expression of IL-2 or IFN-γ measured by intracellular staining in CD4⁺ T cells co-cultured with untreated or TGF-β pre-incubated OCI-Ly10 or OCI-Ly19 cells. (D) Representative histograms (n = 3) showing proliferation of CD4⁺ T cells co-cultured with untreated, TGF-β pre-incubated OCI-Ly19 cells with or without stripping by low pH NaCl/citrate buffer measured by CFSE staining. Proliferative capacity was expressed by calculating the number of CFSE^dim cells. (E) Representative dot plots (n = 3) showing the expression of IL-2 or IFN-γ or IL-17 in CD4⁺ T cells co-cultured with untreated or TGF-β pre-incubated OCI-Ly19 cells with or without stripping by low pH NaCl/citrate buffer measured by intracellular staining. (F) Graphs from a representative lymphoma patient sample showing the expression of IL-2, IFN-γ, CD25 and Ki-67 in CD4⁺ T cells pre-treated with anti-TGF-βR (TβR) and then cocultured with lymphoma B cells (LB) in anti-CD3-coated plate for 3 days. The expression of IL-2, IFN-γ, CD25 and Ki-67 in CD4⁺ T cells was determined by flow cytometry and expressed as a percentage of the total cells. Untreated T cells (U) were used as a control.