Table 1.

Sequence of primers used to amplify amastin isoforms ORFs.

Primer name / gene ID	Primer Sequence (5’-3’)	Restriction enzyme
pδ1-amastin (F) Tc00.1047053511071.40	TTGTTCTAGAGTAGGAAGCAATG	XbaI
pδ1-amastin (R) Tc00.1047053511071.40	CGCTGGATCCGAACCACGTGCA	BamHI
β1-amastin (F) Tc00.1047053509965.390	CCTAGGAGGATGTCGAAGAAGAAG	AvrII
β1-amastin (R) Tc00.1047053509965.390	AGATCTCGAGCACAATGAGGCCCAG	BglII
β2-amastin (F) Tc00.1047053509965.394	TCTAGATGGGCTTCGAAACGCTTGC	XbaI
β2-amastin (R) Tc00.1047053509965.394	GGATCCCCAGTGCCAGCAAGAAGACTG	BamHI

The underlined sequences correspond to the restriction sites recognized by the restriction enzyme.