Table 2.

Minimal intervention sets to prevent binding of Grb2 to Shc in response to insulin stimulation

	Minimal intervention set	Interpretation
1	ir.res_ir=0
2	grb2.res_grb2=0	set basal activity of insulin receptor, Grb2 or Shc to 0, i.e. remove respective species from the system
3	shc.res_shc=0
4	ins.b1=0	prevent insulin binding to its receptor by blocking the binding site on insulin or by blocking both
5	ir.b_ins=0, ir.b_ins2=0	binding sites on the receptor
6	shc.b_ir=0	prevent Shc binding to insulin receptor by blocking the binding site on Shc or by preventing the
7	ir.p1=0	necessary phosphorylation of the receptor
8	grb2.b_shc=0	prevent Grb2 binding to Shc by blocking the binding site on Grb2 or by preventing the
9	shc.p1=0	necessary phosphorylation of Shc

The nine minimal intervention sets preventing binding of Grb2 to Shc in response to insulin stimulation are given in the rows. The sets are grouped according to their effect on the signaling pathway.