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. 2013 Mar 15;8(3):e59602. doi: 10.1371/journal.pone.0059602

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© 2013 Gu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Spleen weight (n = 5–6/group). B. Total number of splenocytes, absolute numbers of T cells, CD4⁺ T cells, CD8⁺ T cells, Tregs, B cells, NK cells, monocytes, CD4⁺/CD8⁺ and Foxp3⁺/Foxp3⁻ ratio in CD4⁺T cells in WT SD rats (n = 5–11/group). C. Total number of splenocytes and absolute numbers of B cells, NK cells as well as monocytes in nude rats (n = 5–8/group). Spleen was collected 3 d after stroke and total splenocytes isolated for immunostaining. Relative percentages of each cell type were obtained from the FACS analyses using splenocytes. Absolute cell numbers were calculated by multiplying the percentage of each cell type with the total cell number in an analyzed sample, and adjusted to a number in 1 spleen. *, **, *** vs. stroke, p<0.05, 0.01, 0.001, respectively.