Table 1.
Differentially expressed (>2 log2) genes in HepG2 cells after treatment with Padma 28
| |
|
Microarray (n=1) |
qPCR (n=5) |
||
|---|---|---|---|---|---|
| Gene | Gene title | log2 (ratio) | Fold | Fold | P(H1) |
| CYP1A1 |
cytochrome P450, family 1, subfamily A1 |
8.7 |
408.4 |
123.4 ± 31.1 |
0.001 |
| PTGR1 |
prostaglandin reductase 1 |
4.8 |
27.4 |
|
|
| SLC7A11 |
solute carrier family 7, member 11 |
4.2 |
18.9 |
|
|
| AKR1B10 |
aldo-keto reductase family 1, member B10 |
3.9 |
14.7 |
13.9 ± 4.9 |
0.003 |
| ASNS |
asparagine synthetase |
3.4 |
10.2 |
18.8 ± 5.5 |
0.008 |
| CYP24A1 |
cytochrome P450, family 24, subfamily A 1 |
3.1 |
8.4 |
5.0 ± 0.9 |
0.132 |
| PMAIP1 |
phorbol-12-myristate-13-acetate-induced protein 1 |
3.0 |
8.2 |
|
|
| NUPR1 |
nuclear protein 1 |
2.6 |
6.3 |
|
|
| SCHIP1 |
schwannomin interacting protein 1 |
2.6 |
6.2 |
|
|
| GSTA1* |
glutathione S-transferase alpha 1 |
2.6 |
6.1 |
4.5 ± 1.3 |
0.036 |
| FECH |
ferrochelatase |
2.4 |
5.2 |
3.7 ± 0.8 |
0.018 |
| CD14 |
CD14 molecule |
2.4 |
5.2 |
|
|
| AKR1C2** |
aldo-keto reductase family 1, member C2 |
2.4 |
5.1 |
6.7 ± 1.2 |
0.007 |
| LOC374443 |
CLR pseudogene |
2.3 |
5.0 |
|
|
| BCAT1 |
branched chain aminotransferase 1, cytosolic |
2.3 |
5.0 |
|
|
| KLHDC9 |
kelch domain containing 9 |
2.2 |
4.6 |
|
|
| HELLS |
helicase, lymphoid-specific |
2.2 |
4.4 |
|
|
| CARS |
cysteinyl-tRNA synthetase |
2.1 |
4.3 |
3.2 ± 0.6 |
0.001 |
| AKR1C1** |
aldo-keto reductase family 1, member C1 |
2.1 |
4.2 |
6.7 ± 1.2 |
0.007 |
| ABCC4 |
ATP-binding cassette, sub-family C, member 4 |
2.1 |
4.2 |
|
|
| PIR |
pirin (iron-binding nuclear protein) |
2.1 |
4.2 |
|
|
| RAB27A |
RAB27A, member RAS oncogene family |
2.1 |
4.2 |
|
|
| HMOX1 |
haeme oxygenase (decycling) 1 |
2.1 |
4.2 |
5.3 ± 0.8 |
0.008 |
| GCLM |
glutamate-cysteine ligase, modifier subunit |
2.1 |
4.1 |
|
|
| RBM39 |
RNA binding motif protein 39 |
-2.8 |
-7.2 |
1.1 ± 0.6 |
0.083 |
| DKK1 |
dickkopf homolog 1 (Xenopus laevis) |
-2.7 |
-6.7 |
-24.2 ± 5.9 |
0.005 |
| SCN1A |
sodium channel, voltage-gated, type I, alpha subunit |
-2.6 |
-6.0 |
-30.3 ± 10 |
0.002 |
| SLC13A3 |
solute carrier family 13, member A3 |
-2.4 |
-5.2 |
|
|
| MT1M |
metallothionein 1M |
-2.3 |
-4.9 |
|
|
| LGR5 |
leucine-rich G protein-coupled receptor 5 |
-2.1 |
-4.4 |
-8.1 ± 1.9 |
0.001 |
| SLC26A3 |
solute carrier family 26, member 3 |
-2.1 |
-4.2 |
|
|
| SH3PXD2A |
SH3 and PX domains 2A |
-2.0 |
-4.1 |
|
|
| SUCLG1 | succinate-CoA ligase, alpha subunit | -2.0 | -4.1 | 0.3 ± 0.6 | 0.786 |
*primers detect GSTA1-A3 and A5; **primers detect AKR1C1-C4a.
The P(H1) value calculated for the qPCR experiments indicates the probability that the difference between sample and control group is significant.