Table 2.
Top canonical pathways and corresponding genes modulated by Padma 28 in HepG2 cells
| |
|
Microarray (n=1) |
RT-PCR (n=5) |
||
|---|---|---|---|---|---|
| Pathway/Gene | Gene title | log2 (ratio) | Fold | Fold | P(H1) |
|
Metabolism of xenobiotics by cytochrome P450
(p-value: 3.82E-05; ratio: 10/178) | |||||
| CYP1A1 |
cytochrome P450, family 1, subfamily A1 |
8.7 |
408.4 |
123.4 ± 31.1 |
0.001 |
| PTGR1 |
prostaglandin reductase 1 |
4.8 |
27.9 |
|
|
| GSTA1 |
glutathione S-transferase alpha 1 |
2.6 |
6.1 |
4.5 ± 1.3 |
0.036 |
| AKR1C2 |
aldo-keto reductase family 1, member C2 |
2.4 |
5.3 |
6.7 ± 1.2 |
0.007 |
| AKR1C1 |
aldo-keto reductase family 1, member C1 |
2.1 |
4.3 |
6.7 ± 1.2 |
0.007 |
| CYP4F11 |
cytochrome P450, family 4, subfamily F 11 |
1.7 |
3.2 |
|
|
| MGST1 |
microsomal glutathione S-transferase 1 |
1.6 |
3.0 |
|
|
| ALDH1L1 |
aldehyde dehydrogenase 1 family, member L1 |
1.6 |
3.0 |
4.1 ± 1.0 |
0.003 |
| EPHX1 |
epoxide hydrolase 1, microsomal |
1.3 |
2.5 |
|
|
| CYP2S1 |
cytochrome P450, family 2, subfamily S1 |
1.2 |
2.2 |
5.1 ± 1.5 |
0.018 |
|
Glutathione metabolism (p-value: 4,01E-04;
ratio 7/92) | |||||
| GSTA1 |
glutathione S-transferase alpha 1 |
2.6 |
6.1 |
4.5 ± 1.3 |
0.036 |
| GCLM |
glutamate-cysteine ligase, modifier subunit |
2.1 |
4.1 |
|
|
| GCLC |
glutamate-cysteine ligase, catalytic subunit |
1.7 |
3.3 |
5.8 ± 0.9 |
0.009 |
| MGST1 |
microsomal glutathione S-transferase 1 |
1.6 |
3.0 |
|
|
| GPX2 |
glutathione peroxidase 2 |
1.2 |
2.3 |
0.3 ± 0.9 |
0.881 |
| RAB15 |
RAB15, member RAS onocogene family |
1.1 |
2.1 |
|
|
| IDH3A |
isocitrate dehydrogenase 3 (NAD+) alpha |
1.1 |
2.1 |
|
|
|
Aminoacyl-tRNA biosynthesis (p-value:
4.24E-04; ratio 6/83) | |||||
| CARS |
cysteinyl-tRNA synthetase |
2.1 |
4.3 |
3.2 ± 0.6 |
0.001 |
| YARS |
tyrosyl-tRNA synthetase |
1.3 |
2.5 |
|
|
| WARS |
tryptophanyl-tRNA synthetase |
1.2 |
2.4 |
3.8 ± 1.0 |
0.020 |
| GARS |
glycyl-tRNA synthetase |
1.2 |
2.3 |
|
|
| AARS |
alanyl-tRNA synthetase |
1.2 |
2.3 |
|
|
| MARS |
methionyl-tRNA synthetase |
1.1 |
2.2 |
|
|
|
LPS/IL-1 mediated inhibition of RXR function
(p-value: 1.82E-03; ratio 12/205) | |||||
| GSTA1 |
glutathione S-transferase alpha 1 |
2.6 |
6.1 |
4.5 ± 1.3 |
0.036 |
| CD14 |
CD14 molecule |
2.4 |
5.3 |
|
|
| ABCC4 |
ATP-binding cassette, sub-family C, member 4 |
2.1 |
4.3 |
|
|
| ABCC3 |
ATP-binding cassette, sub-family C, member 3 |
1.8 |
3.5 |
|
|
| MGST1 |
microsomal glutathione S-transferase 1 |
1.6 |
3.0 |
|
|
| ALDH1L1 |
aldehyde dehydrogenase 1 family, member L1 |
1.6 |
3.0 |
4.1 ± 1.0 |
0.003 |
| NR1H3 |
nuclear receptor subfamily 1, group H, member 3 |
1.5 |
2.8 |
|
|
| ALDH1A1 |
aldehyde dehydrogenase 1 family, member A1 |
1.3 |
2.5 |
|
|
| SLC35A2 |
solute carrier family 35, member A2 |
1.1 |
2.1 |
|
|
| SULT1E1 |
sulfotransferase family 1E, member 1 |
-1.3 |
-2.5 |
|
|
| SLCO1B3 |
solute carrier organic anion transporter 1B3 |
-1.3 |
-2.5 |
|
|
| FMO5 |
flavin containing monooxygenase 5 |
-1.9 |
-3.7 |
|
|
|
Nrf2-mediated oxidative stress response
(p-value: 2.92E-03; ratio 11/181) | |||||
| GSTA1 |
glutathione S-transferase alpha 1 |
2.6 |
6.1 |
4.5 ± 1.3 |
0.036 |
| HMOX1 |
heme oxygenase (decycling) 1 |
2.1 |
4.3 |
5.3 ± 0.8 |
0.008 |
| GCLM |
glutamate-cysteine ligase, modifier subunit |
2.1 |
4.3 |
|
|
| GCLC |
glutamate-cysteine ligase, catalytic subunit |
1.7 |
3.3 |
5.8 ± 0.9 |
0.009 |
| NQO1 |
NAD(P)H dehydrogenase, quinone 1 |
1.7 |
3.2 |
|
|
| MGST1 |
microsomal glutathione S-transferase 1 |
1.6 |
3.0 |
|
|
| EPHX1 |
epoxide hydrolase 1, microsomal (xenobiotic) |
1.3 |
2.5 |
|
|
| FTH1 |
ferritin, heavy polypeptide 1 |
1.3 |
2.5 |
|
|
| TXNRD1 |
thioredoxin reductase 1 |
1.3 |
2.5 |
|
|
| GPX2 |
glutathione peroxidase 2 (gastrointestinal) |
1.2 |
2.3 |
0.3 ± 0.9 |
0.881 |
| SLC35A2 |
solute carrier family 35 , member A2 |
1.1 |
2.1 |
|
|
|
Aryl Hydrocarbon Receptor Signaling
(p-value: 4.65E-03; ratio 9/150) | |||||
| CYP1A1 |
cytochrome P450, family 1, subfamily A1 |
8.7 |
408.4 |
123.4 ± 31.1 |
0.001 |
| GSTA1 |
glutathione S-transferase alpha 1 |
2.6 |
6.1 |
4.5 ± 1.3 |
0.036 |
| NQO1 |
NAD(P)H dehydrogenase, quinone 1 |
1.7 |
3.2 |
|
|
| FAS |
Fas (TNF receptor superfamily, member 6) |
1.7 |
3.2 |
|
|
| MGST1 |
microsomal glutathione S-transferase 1 |
1.6 |
3.0 |
|
|
| ALDH1L1 |
aldehyde dehydrogenase 1 family, member L1 |
1.6 |
3.0 |
4.1 ± 1.0 |
0.003 |
| ALDH1A1 |
aldehyde dehydrogenase 1 family, member A1 |
1.3 |
2.5 |
|
|
| SLC35A2 |
solute carrier family 35, member A2 |
1.1 |
2.1 |
|
|
| NCOA2 | nuclear receptor coactivator 2 | -1.1 | -2.1 | ||
The p-value is a measure of the probability of the association of a specific pathway and the dataset. The ratio indicates the number of molecules in a given pathway from the input dataset that meet the cut-off criteria (fold change of 2) divided by the number of molecules that make up that pathway in the database.