Table 2. Characteristics of 15 microsatellite markers developed for Bursaphelenchus xylophilus.
Locus | Primer sequence (5' - 3') | Motif repeat | Fluorescent label/Multiplex panel | Allele size range (bp) | Accession Number |
PWN_3 | F : GAAATCTGGGGAGCAAAACA | (CT)8 | 6FAM/MC33 | 215–227 | HF563643 |
R : ACCGCACTCGCACTTAGATT | |||||
PWN_6 | F : GGAATTAGGCGTCCACAAGA | (AG)7 | 6FAM/MC33 | 126–131 | HF563644 |
R : TGCTGTATAAACATTTGCTCTTCG | |||||
PWN_26 | F : GAAAAACTTAGGCTGGGGGA | (TG)5 | PET/MA28 | 157–160 | HF563645 |
R : TAGTGACGACTCATCCGCTG | |||||
PWN_30 | F : ACCTAGCGTCGAAAACCCTT | (TG)5 | VIC/MB28 | 207–209 | HF563646 |
R : ATAGCAGCAGGTCAAATCCG | |||||
PWN_34 | F : CCATTGCCCAAAGGATTAAA | (CT)7 | PET/MC33 | 82–95 | HF563647 |
R : ACGTAGCATTCGGAGTGACC | |||||
PWN_35 | F : ACCGCCTGGTAACCGAGT | (GA)6 | PET/MB28 | 185–193 | HF563648 |
R : TTGGACACTGCGAGTAAGGA | |||||
PWN_49 | F : CTGGGAGTTCTTTTTGCTCG | (AAC)5 | PET/MA28 | 174–177 | HF563649 |
R : GCAACAATCGTTAGTGGCAA | |||||
PWN_51 | F : GGAAGAGACTTGACCCGAAA | (AG)7 | 6FAM/MC33 | 84–96 | HF563650 |
R : GGAAAAGAGTCCTCACGTCAA | |||||
PWN_54 | F : ACCTTCACACTTGTAGCCGC | (AG)7 | PET/MA28 | 113–119 | HF563651 |
R : CCGGTCATCATAATCTCTGATCT | |||||
PWN_56 | F : TCTTCACATTAATCTTGCTGCC | (CA)8 | PET/MC33 | 185–195 | HF563652 |
R : AACGATTAGGAACGCAGTGG | |||||
PWN_60 | F : GGCGAAACGGATAAAGGAAT | (CA)9 | VIC/MB28 | 129–147 | HF563653 |
R : TTCTTCCCCAAACCTTCTCC | |||||
PWN_62 | F : GAGCTATAGCCCCTGCCTTT | (CT)6 | 6FAM/MA28 | 112–124 | HF563654 |
R : AGCCTTGCGAAGAAACAAAA | |||||
PWN_79 | F : TGGATACAAACGGTTGAGGA | G(GA)2G(GA)8A(T)5 | NED/MB28 | 107–114 | HF563655 |
R : AACCTCATCTGTCCGTGGAT | |||||
PWN_80 | F : AATTGGTGCTCCTGTATGGC | TG(TGT)5TG | VIC/MB28 | 78–88 | HF563656 |
R : CGGCTTACTCTTTGTCCCAA | |||||
PWN_84 | F : CCGTGTTTTCAACTCATTCC | (CT)2T(CT)5C | PET/MC33 | 129–137 | HF563657 |
R : TTTGATCCGATTACCTTCGG | |||||
Bx07 | F : AACGGAAAAGAGTCCTCACG | (TC)10 | 6FAM/MB28 | 146–157 | [22] |
R : TAGGCCCTCCTTGACAAAAGC | |||||
Bx08 | F : CTGCCTATTTTCGACTTCTC | (CT)10 | NED/MA28 | 105–113 | [22] |
R : CAAGGATCGTGTTCCTCTTTTTG |
Characteristics of the microsatellite markers from Zhou et al. [22], Bx07 and Bx08, are also given.