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. 2013 Mar 15;8(3):e59165. doi: 10.1371/journal.pone.0059165

Table 2. Characteristics of 15 microsatellite markers developed for Bursaphelenchus xylophilus.

Locus Primer sequence (5' - 3') Motif repeat Fluorescent label/Multiplex panel Allele size range (bp) Accession Number
PWN_3 F : GAAATCTGGGGAGCAAAACA (CT)8 6FAM/MC33 215–227 HF563643
R : ACCGCACTCGCACTTAGATT
PWN_6 F : GGAATTAGGCGTCCACAAGA (AG)7 6FAM/MC33 126–131 HF563644
R : TGCTGTATAAACATTTGCTCTTCG
PWN_26 F : GAAAAACTTAGGCTGGGGGA (TG)5 PET/MA28 157–160 HF563645
R : TAGTGACGACTCATCCGCTG
PWN_30 F : ACCTAGCGTCGAAAACCCTT (TG)5 VIC/MB28 207–209 HF563646
R : ATAGCAGCAGGTCAAATCCG
PWN_34 F : CCATTGCCCAAAGGATTAAA (CT)7 PET/MC33 82–95 HF563647
R : ACGTAGCATTCGGAGTGACC
PWN_35 F : ACCGCCTGGTAACCGAGT (GA)6 PET/MB28 185–193 HF563648
R : TTGGACACTGCGAGTAAGGA
PWN_49 F : CTGGGAGTTCTTTTTGCTCG (AAC)5 PET/MA28 174–177 HF563649
R : GCAACAATCGTTAGTGGCAA
PWN_51 F : GGAAGAGACTTGACCCGAAA (AG)7 6FAM/MC33 84–96 HF563650
R : GGAAAAGAGTCCTCACGTCAA
PWN_54 F : ACCTTCACACTTGTAGCCGC (AG)7 PET/MA28 113–119 HF563651
R : CCGGTCATCATAATCTCTGATCT
PWN_56 F : TCTTCACATTAATCTTGCTGCC (CA)8 PET/MC33 185–195 HF563652
R : AACGATTAGGAACGCAGTGG
PWN_60 F : GGCGAAACGGATAAAGGAAT (CA)9 VIC/MB28 129–147 HF563653
R : TTCTTCCCCAAACCTTCTCC
PWN_62 F : GAGCTATAGCCCCTGCCTTT (CT)6 6FAM/MA28 112–124 HF563654
R : AGCCTTGCGAAGAAACAAAA
PWN_79 F : TGGATACAAACGGTTGAGGA G(GA)2G(GA)8A(T)5 NED/MB28 107–114 HF563655
R : AACCTCATCTGTCCGTGGAT
PWN_80 F : AATTGGTGCTCCTGTATGGC TG(TGT)5TG VIC/MB28 78–88 HF563656
R : CGGCTTACTCTTTGTCCCAA
PWN_84 F : CCGTGTTTTCAACTCATTCC (CT)2T(CT)5C PET/MC33 129–137 HF563657
R : TTTGATCCGATTACCTTCGG
Bx07 F : AACGGAAAAGAGTCCTCACG (TC)10 6FAM/MB28 146–157 [22]
R : TAGGCCCTCCTTGACAAAAGC
Bx08 F : CTGCCTATTTTCGACTTCTC (CT)10 NED/MA28 105–113 [22]
R : CAAGGATCGTGTTCCTCTTTTTG

Characteristics of the microsatellite markers from Zhou et al. [22], Bx07 and Bx08, are also given.