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. 2013 Feb 4;13:20. doi: 10.1186/1471-2229-13-20

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Copyright ©2013 Barbez et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cellular localization and trafficking of PIN1-RFP. (A) Colocalization of PIN1 proteins (in red) with the ER marker HDEL-GFP (in green) 10 h after transformation. (B) PIN1-RFP preferentially localizes to the plasma membrane (PM) after 16 h. (C) 24 h after transformation, PIN1-RFP shows vacuolar localization indicating lytic turnover. (D) Relative number of cells showing pronounced PIN1-RFP signal in the ER, PM, and in the vacuole over a time course of 24 h after transformation. Cells showing PIN1-RFP both at PM and either at the ER or vacuole were ascertained as ER or vacuole positive, respectively. Bars = 10 μm.