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Summary of the optimized process used in this study for tyrosinase production
| Parameters | Tyrosinase production |
|---|---|
| Strain |
JM109 (pMFvpt) |
| Medium |
Minimal medium containing glycerol and NZ-amine |
| Temperature (°C) |
32 |
| pH |
6.9 |
| Dissolved oxygen (%) |
30 |
| Cultivation |
Fed-batch culture with exponential feeding |
| Feeding medium |
Glycerol and NZ-amine |
| Feeding rate (h-1) |
0.3 |
| Induction stage |
At OD600 value of 30-40 |
| Harvest | End of the exponential growth |
