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. 2013 Mar 15;8(3):e58795. doi: 10.1371/journal.pone.0058795

Figure 5. Transcriptional activity of PLCz promoter with recombinant haplotypes in MLTC-1 cells.

Figure 5

A. 5′-flanking region of bovine PLCz gene, as identified using the NCBI database. The base numbers are relative to the A of TSS. The red box color represents exon 1. g.−456 G>A and g. +65 T>C are located in the 5′-flanking region (−2290 nt to +112 nt) of PLCz. Other numbers represent primer positions for the cloning reporter constructs. B. TA1 fragments with different haplotypes (H2, H3, H4, and H1) were amplified by PCR to generate the reporter constructs; the various recombinant haplotypes are shown above the line. Each fragment was cloned into the pGL3 basic vector and transfected into MLTC-1 cells. C. Transcriptional activities of the PLCz promoter with various haplotypes were measured by dual luciferase assays. For each construct, individual plasmid DNA extracted from 6 to 9 colonies was used. Results are presented as the average fold change of firefly luciferase activity versus the Renilla control vector (mean±S.D., n = 6 to 9) (* indicates P<0.05, ** indicates P<0.01 vs. H2).