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. 2013 Mar 15;8(3):e58866. doi: 10.1371/journal.pone.0058866

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© 2013 Bowen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The measured currents in wild type HEK-293 cells are inductive of no over-expression of TRPV6 channels. For TRPV6/EGFP HEK-293, the absence of La³⁺ ions showed a large current with the current being efficiently blocked in the presence of La³⁺ ions. Effect of La³⁺ ions (10 µM) on currents evoked by a 50 ms ramp (bottom) in wild type HEK-293 cells and TRPV6/EGFP HEK-293 cells.