Figure 5.

Ro5-4864 inhibits TNF-α production in response to Ag and LPS. IgE-loaded BMMCs were pretreated with DMSO, Ro5-4864 or clonazepam for 20 min and subsequently stimulated with 20 ng/ml Ag (A) or 5 μg/ml LPS (B) for 3 h or left unstimulated. TNF-α concentrations in the supernatants were determined by ELISA. Each bar is the mean of triplicates ± SEM. Comparable results were obtained with cells from different cultures (n≥3). Marks of significance (“stars”) relate to the respective vehicle (DMSO) control.