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. 2013 Mar 15;8(3):e58828. doi: 10.1371/journal.pone.0058828

Figure 2. Yy1 cKO visceral endoderm displays a reduction of apical lysosome size and other epithelial characteristics.

Figure 2

A–B, E–F) Transmission electron microscopy (TEM) of WT (A–B) and cKO (E–F) visceral endoderm (VE) sections reveals large apical lysosomes in 8.5 and 9.0 dpc WT visceral endoderm (asterisks, A–B) and in 8.5 dpc cKO visceral endoderm (asterisks, E). At 9.0 dpc the size of the apical lysosomes are greatly reduced in the cKO (compare asterisks, in F to B). C–D, G–H) IgG localization (green) at the apical surface is readily noted in 9.0–9.5 dpc WT yolk sac sections (YS, C–D) while IgG distribution is reduced in the mutant at the same stages (G–H). Inset is a higher magnification view of a portion of the visceral endoderm. I–J, M–N). Whole-mount LysoTracker Red staining reveals large filled lysosomes from 9.0–9.5 dpc (I–J) in WT tissue while the LysoTracker-filled areas are reduced in the mutant samples (M–N). K–L, O–P) Immunolocalization of E-Cadherin (CDH1; green) reveals epithelial cell-cell adhesions in the visceral endoderm of WT yolk sac sections from 9.0–9.5 dpc (K–L). CDH1 expression is slightly reduced at 9.0 dpc and more profoundly reduced at 9.5 dpc in cKO visceral endoderm (O–P). ME = mesoderm; N = nucleus.