Skip to main content
. 2013 Mar 15;8(3):e58828. doi: 10.1371/journal.pone.0058828

Figure 7. Inhibition of FLK1 in WT embryos results in yolk sac defects similar to Yy1 cKO.

Figure 7

A–J) WT 8.5 dpc embryos cultured until they reached 9.5 dpc in the absence (A–E; −SU1498) or presence of the small molecule SU1498 (F–J; +SU1498). Compared with control embryos (A–E), SU1498 treated embryos displayed clear yolk sac abnormalities, including pooled blood in the proximal yolk sac (F), a small embryo (G), reduced HNF4α (H), reduced apical IgG localization (I) and higher amounts of cleaved Caspase-3 (CASP3) staining (J). Asterisks in J indicate cleaved Caspase-3 positive cells; dotted line represents the division between the yolk sac mesoderm (ME) and visceral endoderm (VE).