Table 1.
Protein identities and relative changes in their expression between MeJA-treated jin1-9/myc2 and wild-type plants after 6 h or 48 h of MeJA treatment
|
Spot No.a |
Category and name |
gi Numberb |
Exp pI/kDac |
Thero pI/kDad |
Scoree |
SCf(%) |
PNg |
MeJA-Treated WT 6h |
MeJA-Treated jin1-9 6hh |
Fold change |
p-value |
MeJA-Treated WT 48h |
MeJA-Treated jin1-9 48hh |
Fold change |
p-value |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vol% (mean±SD) | Vol% (mean±SD) | Vol% (mean±SD) | Vol% (mean±SD) | ||||||||||||
| |
Glucosinolate metabolism |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 216 |
glutathione S-transferase ERD13 (GSTF10)Neg |
gi|15224582 |
5.82/33.0 |
5.54/29.8 |
66 |
5 |
2 |
0.11±0.00 |
0.17±0.00 |
1.51 |
0.00 |
0.17±0.10 |
0.09±0.09 |
-1.88 |
0.37 |
| 327 |
myrosinase-binding-like protein |
gi|30684083 |
5.14/76.0 |
5.12/48.5 |
131 |
21 |
6 |
0.05±0.01 |
0.04±0.00 |
-1.15 |
0.23 |
0.08±0.02 |
0.04±0.01 |
-2.10 |
0.02 |
| 509 |
sulfotransferase 18 |
gi|15221130 |
5.66/57.0 |
5.52/40.7 |
177 |
13 |
4 |
0.01±0.00 |
0.00±0.00 |
-9999 |
0.01 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| 523 |
3-isopropylmalate dehydrogenaseup |
gi|15241338 |
5.04/58.0 |
5.75/44.2 |
88 |
2 |
1 |
0.08±0.00 |
0.05±0.01 |
-1.63 |
0.00 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| 556 |
amidase 1 |
gi|18390964 |
6.08/61.0 |
5.83/45.4 |
152 |
22 |
6 |
0.02±0.01 |
0.00±0.00 |
-9999 |
0.04 |
0.02±0.03 |
0.00±0.00 |
-9999 |
0.19 |
| 864 |
thioglucoside glucohydrolase 1 (TGG1) |
gi|15809938 |
5.24/121.0 |
5.67/61.7 |
141 |
10 |
4 |
0.02±0.01 |
0.00±0.00 |
-9999 |
0.02 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| |
Stress and defense |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 113 |
glutathione S-transferase DHAR1Pos |
gi|8778432 |
5.66/31.9 |
6.82/50.6 |
150 |
6 |
2 |
0.12±0.03 |
0.07±0.02 |
-1.75 |
0.05 |
0.48±0.42 |
0.31±0.02 |
-1.56 |
0.00 |
| 161 |
putative glyoxalase |
gi|9828630 |
5.08/38.9 |
6.97/40.3 |
85 |
12 |
3 |
0.04±0.02 |
0.00±0.00 |
-9999 |
0.02 |
0.05±0.01 |
0.02±0.01 |
-2.22 |
0.01 |
| 183 |
glutathione S-transferase (AtGSTF3) |
gi|497788 |
6.40/29.1 |
5.80/23.5 |
116 |
11 |
2 |
0.03±0.01 |
0.00±0.00 |
-9999 |
0.00 |
0.08±0.00 |
0.01±0.00 |
-14.16 |
0.00 |
| 203 |
putative arginasePos |
gi|15236635 |
6.01/44.0 |
5.90/38.1 |
163 |
12 |
4 |
0.11±0.01 |
0.13±0.01 |
1.14 |
0.05 |
0.30±0.02 |
0.19±0.03 |
-1.61 |
0.01 |
| 275 |
HSP20-like chaperones protein |
gi|3193303 |
4.17/36.0 |
4.42/28.0 |
103 |
16 |
4 |
0.04±0.01 |
0.00±0.00 |
-9999 |
0.00 |
0.02±0.01 |
0.00±0.00 |
-9999 |
0.09 |
| 338 |
putative plastid-lipid-associated protein 1 |
gi|15233357 |
4.52/41.0 |
5.45/35.0 |
161 |
23 |
5 |
0.02±0.01 |
0.00±0.00 |
-9999 |
0.00 |
0.13±0.08 |
0.03±0.03 |
-4.55 |
0.09 |
| 408 |
O-acetylserine (thiol) lyase B (oasB) |
gi|15224351 |
5.59/46.0 |
8.13/42.0 |
183 |
29 |
8 |
0.02±0.00 |
0.10±0.01 |
4.70 |
0.00 |
0.02±0.01 |
0.00±0.00 |
-9999 |
0.12 |
| 437 |
O-acetylserine (thiol) lyase isoform C (oasC) |
gi|6899947 |
5.75/49.0 |
6.96/41.5 |
96 |
12 |
3 |
0.02±0.01 |
0.00±0.00 |
-9999 |
0.01 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| 872 |
luminal binding protein (BiP2) |
gi|1303695 |
5.16/122.0 |
5.08/73.7 |
165 |
11 |
6 |
0.04±0.01 |
0.03±0.00 |
-1.67 |
0.04 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| |
Photosynthesis |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 126 |
chlorophyll a/b-binding protein |
gi|13265501 |
5.47/23.9 |
6.52/26.1 |
178 |
9 |
3 |
0.12±0.01 |
0.19±0.01 |
1.60 |
0.00 |
0.07±0.00 |
0.06±0.00 |
-1.08 |
0.13 |
| 170 |
chloroplast NAD-MDH |
gi|3256066 |
6.18/39.8 |
8.48/42.6 |
295 |
14 |
5 |
0.17±0.11 |
0.03±0.03 |
-5.88 |
0.10 |
0.13±0.01 |
0.09±0.02 |
-1.55 |
0.03 |
| 233 |
coproporphyrinogen III oxidase |
gi|240254000 |
5.51/50.0 |
6.24/44.1 |
155 |
35 |
9 |
0.07±0.00 |
0.07±0.00 |
-1.02 |
0.14 |
0.10±0.01 |
0.06±0.00 |
-1.62 |
0.01 |
| 247 |
uroporphyrinogen decarboxylase 2 |
gi|15226690 |
6.72/54.0 |
8.29/43.7 |
517 |
32 |
11 |
0.07±0.00 |
0.07±0.00 |
-1.05 |
0.10 |
0.03±0.01 |
0.00±0.00 |
-9999 |
0.01 |
| 346 |
ribulose bisphosphate carboxylase |
gi|1944432 |
5.93/81.0 |
6.12/48.0 |
167 |
16 |
7 |
1.04±0.68 |
0.13±0.09 |
-8.33 |
0.08 |
1.00±0.26 |
0.00±0.00 |
-9999 |
0.00 |
| 865 |
transketolase-like protein |
gi|7329685 |
5.56/120.0 |
5.80/81.9 |
113 |
17 |
8 |
0.08±0.00 |
0.15±0.02 |
1.84 |
0.01 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| |
Carbohydrate metabolism |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 272 |
3-oxoacyl-[acyl-carrier protein] reductase (NADH) |
gi|15229203 |
6.40/36.1 |
6.12/28.2 |
61 |
9 |
2 |
0.03±0.01 |
0.00±0.00 |
-9999 |
0.00 |
0.03±0.00 |
0.03±0.00 |
-1.31 |
0.05 |
| 766 |
2-oxoglutarate dehydrogenase, E3 subunit |
gi|4210334 |
6.40/95.0 |
6.96/54.0 |
137 |
34 |
5 |
0.02±0.00 |
0.00±0.00 |
-9999 |
0.00 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| |
Protein synthesis, folding and destination |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 38 |
40S ribosomal protein S12-1 |
gi|15218373 |
5.05/20.0 |
5.38/15.7 |
73 |
22 |
2 |
0.08±0.01 |
0.02±0.00 |
-3.76 |
0.00 |
0.08±0.01 |
0.03±0.01 |
-2.37 |
0.01 |
| 178 |
ATP-dependent Clp protease proteolytic subunit 2 |
gi|18420643 |
6.40/29.1 |
6.71/26.4 |
92 |
14 |
3 |
0.02±0.01 |
0.00±0.00 |
-9999 |
0.01 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| 799 |
TCP-1/cpn60 chaperonin family protein |
gi|15231255 |
5.19/101.0 |
5.60/63.7 |
308 |
27 |
12 |
0.01±0.01 |
0.00±0.00 |
-9999 |
0.02 |
0.00±0.00 |
0.00±0.00 |
— |
— |
| |
other |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 30 | SRPBCC ligand-binding domain-containing protein | gi|15236566 | 6.41/18.1 | 5.91/17.6 | 72 | 15 | 2 | 0.12±0.08 | 0.11±0.06 | -1.08 | 0.89 | 0.34±0.03 | 0.00±0.00 | -9999 | 0.00 |
a Assigned spot number as indicated in Figure 1. b Database accession numbers according to NCBInr. c Experimental mass (kDa) and pI of identified proteins. d Theoretical mass (kDa) and pI of identified proteins.e Mascot score reported after searching against the NCBInr database. f Sequence coverage. g Number of peptides sequenced. h Spot abundance change is expressed as the ratio of intensities (vol.%) of up-regulated (positive value) or down-regulated (negative value) proteins between MeJA-treated jin1-9 and WT. Neg Negatively regulated by MYC2. Pos Positively regulated by MYC2. up Up-regulated by MeJA. Paired Student's t-test was used to define the significant changes ( p <0.05). -9999 indicates the protein spots were not detectable in jin1-9 after MeJA treatment, short string indicates the protein spots were not detectable in WT and jin1-9 after MeJA treatment. Three treatments were performed.