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. 2012 Sep 25;10:57. doi: 10.1186/1477-5956-10-57

Table 1.

Protein identities and relative changes in their expression between MeJA-treated jin1-9/myc2 and wild-type plants after 6 h or 48 h of MeJA treatment

Spot No.a
 Category and name
 gi Numberb
 Exp pI/kDac
 Thero pI/kDad
 Scoree
 SCf(%)
 PNg
 MeJA-Treated WT 6h
 MeJA-Treated jin1-9 6hh
 Fold change
 p-value
 MeJA-Treated WT 48h
 MeJA-Treated jin1-9 48hh
 Fold change
 p-value
                Vol% (mean±SD) Vol% (mean±SD)     Vol% (mean±SD) Vol% (mean±SD)    
 
 Glucosinolate metabolism
  
  
  
  
  
  
  
  
  
  
  
  
  
  
216
 glutathione S-transferase ERD13 (GSTF10)Neg
 gi|15224582
 5.82/33.0
 5.54/29.8
 66
 5
 2
 0.11±0.00
 0.17±0.00
 1.51
 0.00
 0.17±0.10
 0.09±0.09
 -1.88
 0.37
327
 myrosinase-binding-like protein
 gi|30684083
 5.14/76.0
 5.12/48.5
 131
 21
 6
 0.05±0.01
 0.04±0.00
 -1.15
 0.23
 0.08±0.02
 0.04±0.01
 -2.10
 0.02
509
 sulfotransferase 18
 gi|15221130
 5.66/57.0
 5.52/40.7
 177
 13
 4
 0.01±0.00
 0.00±0.00
 -9999
 0.01
 0.00±0.00
 0.00±0.00
 —
 —
523
 3-isopropylmalate dehydrogenaseup
 gi|15241338
 5.04/58.0
 5.75/44.2
 88
 2
 1
 0.08±0.00
 0.05±0.01
 -1.63
 0.00
 0.00±0.00
 0.00±0.00
 —
 —
556
 amidase 1
 gi|18390964 
 6.08/61.0
 5.83/45.4
 152
 22
 6
 0.02±0.01
 0.00±0.00
 -9999
 0.04
 0.02±0.03
 0.00±0.00
 -9999
 0.19
864
 thioglucoside glucohydrolase 1 (TGG1)
 gi|15809938
 5.24/121.0
 5.67/61.7
 141
 10
 4
 0.02±0.01
 0.00±0.00
 -9999
 0.02
 0.00±0.00
 0.00±0.00
 —
 —
 
 Stress and defense
  
  
  
  
  
  
  
  
  
  
  
  
  
  
113
 glutathione S-transferase DHAR1Pos
 gi|8778432 
 5.66/31.9
 6.82/50.6
 150
 6
 2
 0.12±0.03
 0.07±0.02
 -1.75
 0.05
 0.48±0.42
 0.31±0.02
 -1.56
 0.00
161
 putative glyoxalase
 gi|9828630
 5.08/38.9
 6.97/40.3
 85
 12
 3
 0.04±0.02
 0.00±0.00
 -9999
 0.02
 0.05±0.01
 0.02±0.01
 -2.22
 0.01
183
 glutathione S-transferase (AtGSTF3)
 gi|497788 
 6.40/29.1
 5.80/23.5
 116
 11
 2
 0.03±0.01
 0.00±0.00
 -9999
 0.00
 0.08±0.00
 0.01±0.00
 -14.16
 0.00
203
 putative arginasePos
 gi|15236635
 6.01/44.0
 5.90/38.1
 163
 12
 4
 0.11±0.01
 0.13±0.01
 1.14
 0.05
 0.30±0.02
 0.19±0.03
 -1.61
 0.01
275
 HSP20-like chaperones protein
 gi|3193303
 4.17/36.0
 4.42/28.0
 103
 16
 4
 0.04±0.01
 0.00±0.00
 -9999
 0.00
 0.02±0.01
 0.00±0.00
 -9999
 0.09
338
 putative plastid-lipid-associated protein 1
 gi|15233357
 4.52/41.0
 5.45/35.0
 161
 23
 5
 0.02±0.01
 0.00±0.00
 -9999
 0.00
 0.13±0.08
 0.03±0.03
 -4.55
 0.09
408
 O-acetylserine (thiol) lyase B (oasB)
 gi|15224351 
 5.59/46.0
 8.13/42.0
 183
 29
 8
 0.02±0.00
 0.10±0.01
 4.70
 0.00
 0.02±0.01
 0.00±0.00
 -9999
 0.12
437
 O-acetylserine (thiol) lyase isoform C (oasC)
 gi|6899947
 5.75/49.0
 6.96/41.5
 96
 12
 3
 0.02±0.01
 0.00±0.00
 -9999
 0.01
 0.00±0.00
 0.00±0.00
 —
 —
872
 luminal binding protein (BiP2)
 gi|1303695
 5.16/122.0
 5.08/73.7
 165
 11
 6
 0.04±0.01
 0.03±0.00
 -1.67
 0.04
 0.00±0.00
 0.00±0.00
 —
 —
 
 Photosynthesis
  
  
  
  
  
  
  
  
  
  
  
  
  
  
126
 chlorophyll a/b-binding protein
 gi|13265501 
 5.47/23.9
 6.52/26.1
 178
 9
 3
 0.12±0.01
 0.19±0.01
 1.60
 0.00
 0.07±0.00
 0.06±0.00
 -1.08
 0.13
170
 chloroplast NAD-MDH
 gi|3256066
 6.18/39.8
 8.48/42.6
 295
 14
 5
 0.17±0.11
 0.03±0.03
 -5.88
 0.10
 0.13±0.01
 0.09±0.02
 -1.55
 0.03
233
 coproporphyrinogen III oxidase
 gi|240254000
 5.51/50.0
 6.24/44.1
 155
 35
 9
 0.07±0.00
 0.07±0.00
 -1.02
 0.14
 0.10±0.01
 0.06±0.00
 -1.62
 0.01
247
 uroporphyrinogen decarboxylase 2
 gi|15226690
 6.72/54.0
 8.29/43.7
 517
 32
 11
 0.07±0.00
 0.07±0.00
 -1.05
 0.10
 0.03±0.01
 0.00±0.00
 -9999
 0.01
346
 ribulose bisphosphate carboxylase
 gi|1944432
 5.93/81.0
 6.12/48.0
 167
 16
 7
 1.04±0.68
 0.13±0.09
 -8.33
 0.08
 1.00±0.26
 0.00±0.00
 -9999
 0.00
865
 transketolase-like protein
 gi|7329685
 5.56/120.0
 5.80/81.9
 113
 17
 8
 0.08±0.00
 0.15±0.02
 1.84
 0.01
 0.00±0.00
 0.00±0.00
 —
 —
 
 Carbohydrate metabolism
  
  
  
  
  
  
  
  
  
  
  
  
  
  
272
 3-oxoacyl-[acyl-carrier protein] reductase (NADH)
 gi|15229203
 6.40/36.1
 6.12/28.2
 61
 9
 2
 0.03±0.01
 0.00±0.00
 -9999
 0.00
 0.03±0.00
 0.03±0.00
 -1.31
 0.05
766
 2-oxoglutarate dehydrogenase, E3 subunit
 gi|4210334 
 6.40/95.0
 6.96/54.0
 137
 34
 5
 0.02±0.00
 0.00±0.00
 -9999
 0.00
 0.00±0.00
 0.00±0.00
 —
 —
 
 Protein synthesis, folding and destination
  
  
  
  
  
  
  
  
  
  
  
  
  
  
38
 40S ribosomal protein S12-1
 gi|15218373
 5.05/20.0
 5.38/15.7
 73
 22
 2
 0.08±0.01
 0.02±0.00
 -3.76
 0.00
 0.08±0.01
 0.03±0.01
 -2.37
 0.01
178
 ATP-dependent Clp protease proteolytic subunit 2
 gi|18420643 
 6.40/29.1
 6.71/26.4
 92
 14
 3
 0.02±0.01
 0.00±0.00
 -9999
 0.01
 0.00±0.00
 0.00±0.00
 —
 —
799
 TCP-1/cpn60 chaperonin family protein
 gi|15231255
 5.19/101.0
 5.60/63.7
 308
 27
 12
 0.01±0.01
 0.00±0.00
 -9999
 0.02
 0.00±0.00
 0.00±0.00
 —
 —
 
 other
  
  
  
  
  
  
  
  
  
  
  
  
  
  
30 SRPBCC ligand-binding domain-containing protein gi|15236566 6.41/18.1 5.91/17.6 72 15 2 0.12±0.08 0.11±0.06 -1.08 0.89 0.34±0.03 0.00±0.00 -9999 0.00

a Assigned spot number as indicated in Figure 1. b Database accession numbers according to NCBInr. c Experimental mass (kDa) and pI of identified proteins. d Theoretical mass (kDa) and pI of identified proteins.e Mascot score reported after searching against the NCBInr database. f Sequence coverage. g Number of peptides sequenced. h Spot abundance change is expressed as the ratio of intensities (vol.%) of up-regulated (positive value) or down-regulated (negative value) proteins between MeJA-treated jin1-9 and WT. Neg Negatively regulated by MYC2. Pos Positively regulated by MYC2. up Up-regulated by MeJA. Paired Student's t-test was used to define the significant changes ( p <0.05). -9999 indicates the protein spots were not detectable in jin1-9 after MeJA treatment, short string indicates the protein spots were not detectable in WT and jin1-9 after MeJA treatment. Three treatments were performed.