Figure 5.
ABT-737 and chloroquine combination therapy alters the expression levels of apoptotic regulatory factors in SCLC. The cell lines H82, H209 and H345 were treated with DMSO (control), ABT-737, CQ, and combinations of the two drugs for 72 hours before protein lysates were isolated. Doses of ABT-737 and CQ that were below or approximating the cell line specific IC50 values for each agent were chosen. Beta-actin is shown as an internal loading control and cleaved-PARP is shown for relative induction of apoptosis. NOXA was undetectable in H209 and Bcl-2 was barely detectable in H82.