Table 2.
Sense and antisense primers for amplification of the tested genes with real-time PCR
| mRNA target | Sense (5′) primers | Antisense (3′) primers |
|---|---|---|
| xIAP |
5′-CCATATACCCGAGGAACCCT-3′ |
5′-TTTCCACCACAACAAAAGCA-3′ |
| Mcl-1 |
5′-AAAAGCAAGTGGCAAGAGGA-3′ |
5′-TTAATGAATTCGGCGGGTAA-3′ |
| Bcl-2 |
5′-AAGATTGATGGGATCGTTGC-3′ |
5′-TGTGCTTTGCATTCTTGGAC-3′ |
| cIAP-1 |
5′-GCTCAGTAACTGGGAACCAAA-3′ |
5′-ATCATTGCGACCCACATAATA-3′ |
| Survivin |
5′- CAGATTTGAATCGCGGGACCC-3′ |
5′- CCAAGTCTGGCTCGTTCTCAG-3′ |
| IGF-1R |
5′-TTAAAATGGCCAGAACCTGAG-3′ |
5′-ATTATAACCAAGCCTCCCAC-3′ |
| PIK3CD |
5′-CGGGACACAGGGAAGTTCAGGT-3′ |
5′-TAAGGAGTCAGGCCAGGGCGG-3′ |
| 18 s rRNA | 5′-GTAACCCGTTGAACCCCATT-3′ | 5′-CCATCCAATCGGTAGTAGCG-3′ |