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Western blot of PrPScin brain homogenate from RML scrapie-affected C57Bl/6 mice treated with 2.5% SDS for 30, 90, or 180 minutes. PrPSc-immunoreactivity was decreased but still detectable after PK digestion of samples exposed to SDS for 30 (lane 3), 90 (lane 4), or 180 minutes (lane 5). Lane 1, molecular weight marker (kDa); lane 2, RML positive control.
