Table 4.
List of primers used for the Real-Time PCR analysis
| Gene name | GI | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|---|
|
Actin |
99030435 |
TGCTGAGCGAGAAATTGTCAGGG |
TTCCATGCCAATGAAGGATGGCT |
|
APX |
600115 |
ATCGCCGAGAAGAATTGCG |
GGTTCTTCATGGTGCCGAA |
|
PPi |
195610705 |
GAGCTCTCGTTGGCCTGATTT |
ACGAAGAAGCATGGTCACAGC |
|
V-PPi |
238011099 |
GTGTTGCAATTGGTCTGTGG |
CTGCAAGAATCTGCAACGTC |
|
pDIR9 |
226498795 |
CCACTTCTTCTTCCACGACAC |
TCCATCACGTTCACCATCC |
|
PPIase |
226500875 |
AGCTTTGCACCAAGGTTCTG |
TCAGGATCGATTTCCAGTGC |
| IcoA DH | 195642911 | ATGTCGTCAGCATGAAGTGC | CGTAAACAACCAGTGTCTGAGC |
Inorganic pyrophosphate (PPi), vacuolar inorganic pyrophosphate (V-PPi), ascorbate peroxidase (APX), isovaleryl-CoA dehydrogenase (ICoA DH), peptidyl-prolyl isomerase (PPIase).