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Localisation of CotZ-UreA and CotB-GGGEAAAKGGG-UreA fusion proteins as assessed by immunofluorescence microscopy. Purified, free spores of wild type strain 168, BKH102 (cotZ-ureA) and BKH124 (cotB-GGGEAAAKGG-ureA) were visualised by phase contrast (PC) and immunofluorescence (IF) microscopy. The spores were incubated with mouse anti-UreA antibodies, followed by anti-mouse IgG-Cy3 conjugates. The same exposure time was used for IF images.
