Table 1.

Oligonucleotide list

Name	Sequence (5’-3’)	Restriction site
cotY-F	GCT TAG GAT CCA TGA TGA TGT GTA CGA TTG	BamHI
cotZ-R	CGT AGC GAA TTC AGT TAT CAC TCT TGT CCT C	EcoRI
Linker-gggs-F	CCG GAA TTC ACG GAT TAG GCC GTT TGT CCT CAT GGA CCC GTA TAA AAA GAA TGA TAT TGA GCG TTT TGA CCG TGA G	EcoRI
Linker-gggs-R	CGC GAG CTC TGC GCG GCC GCT AGA GCC ACC GCC ACC GTA GGA TCC GGA TGA TTG ATC ATC TGA AGA TTT TAG TGA TCG TTT AGA TG	SacI
linker-gggeaaakggg-F	GCC TGT TAG GAA TTC CGC TCC AAT CTC TTT TTA CAA TAG AAT ATA TGG AAC CGA AAA TCA TGG CGA TGT ATG AAC GGA TTA GGC C	EcoRI
linker- gggeaaakggg- R	CGC GGA TCC TCC TCC ACC TTT CGC TGC TGC TTC TCC TCC ACC GGA TGA TTG ATC ATC TGA AG	BamHI
ureA-F	GAG GGA TCC ATG AAA CTC ACC CCA AAA G	BamHI
ureA-R	CGC GAG CTC TAG GGC CAT ACA TAG AAA C	SacI

In bold are the recognition sites for the restriction enzymes indicated in the table.