Table 2.
Densitometric analysis
| UreA source | Amount of protein used (ng) | Density in OD/mm2 (standard deviation) | UreA concentration (ng) in extracts (% of total) | n° of recombinant molecules extracted from each spore |
|---|---|---|---|---|
|
Fusion 1 |
|
|
|
|
| Purified UreA |
25.0 ng |
177.8 (± 0.03) |
NA |
|
| 12.5 ng |
85,7 (± 0.09) |
NA |
||
| 6.25 ng |
41.6 (± 0.08) |
NA |
||
| KH102 (CotZ-UreA) |
10.00 μg |
17,4 (± 0,03) |
2.44 (0.02) |
2.5 × 102 |
| 5.00 μg |
8,6 (± 0,01) |
1.20 (0.02) |
||
| |
2.50 μg |
4,4 (± 0,08) |
0.61(0.02) |
|
|
Fusion 2 |
|
|
|
|
| Purified UreA |
25.0 ng |
121.4 (± 0.02) |
NA |
|
| 12.5 ng |
61,3 (± 0.02) |
NA |
||
| 6.25 ng |
30.8 (± 0.05) |
NA |
||
| KH124 (CotB-linker -UreA |
1.250 μg |
67.2 (± 0.03) |
13.83 (1.10) |
1.0 × 104 |
| 0.625 μg |
34.5 (± 0.09) |
7.10 (1,14) |
||
| 0.312 μg | 15.6 (± 0.08) | 3.21 (1.04) |
The average amount of protein extracted from a single spore was 18.6 pg and 20.9 pg for BKH102 and BKH124 respectively.