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. 2013 Feb 2;8:8. doi: 10.1186/1750-1326-8-8

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Copyright ©2013 Fanelli et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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GFAP protein levels and distribution in the hippocampus of WT and Tg mice. (a) GFAP immunohistochemical localization in CA1 hippocampal field of 9-, 12-, and 18-month-old WT and Tg mice. or, stratum oriens; pyr, stratum pyramidale; rad, stratum radiatum. Scale bars, 25 μm. (b) Densitometric analysis of GFAP WB performed on hippocampal protein extracts of 3-, 6-, 9-, 12-, and 18-month-old mice. Values are expressed as mean ± SD. **P < 0.01; ***P < 0.001. (c) Double immunofluorescence of PMP70 (green) in combination with GFAP (red) in the CA1 hippocampal field of 18-month-old WT and Tg brain. Several PMP70⁺/GFAP⁺ cells (yellow) are especially numerous in the pathological genotype. Scale bars, 25 μm.