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. 2013 Feb 5;13:9. doi: 10.1186/1475-2867-13-9

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Copyright ©2013 Litwiniec et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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a-f Immunolocalization of cyclin D1 in A549 cells after treatment with etoposide - fluorescence microscopic examination (cyclin D1 indirect labeling, secondary antibody was goat anti-mouse IgG-Alexa Fluor 488®). a Control cells, b 0.75 μM etoposide, c 1.5 μM etoposide, d, e, f 3 μM etoposide. Enhanced fluorescence signal in selected cells and cyclin D1 aggregations indicated (arrows, arrowheads). Nuclei were counterstained with DAPI. Results are indicative of three independent experiments. Bar 50 μm.