Figure 6.

Effect of isopropanol wash on measured probe response to target. Targets with were individually hybridized with surface-attached probe using our standard 18-hour protocol, followed by either isopropanol wash (red) or standard wash (blue). Signal response at both 55°C and 60°C are reported. Average background is indicated by the dotted line and perfect match response is shown by the solid line. Error bars represent the standard deviation across the arrays for each probe target duplex set.