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Effect of temperature and pH on the activity of purified BCJ2315 toward mandelonitrile. (a) Optimum temperature: enzyme activity was measured at various temperatures (18°C to 70°C) in 100 mM of sodium phosphate buffer (pH 7.0). (b) Optimum pH: enzyme activity was measured in different buffers (pH 4.0 to 10.6) at 30°C. The relative activity was expressed as a percentage of the maximum activity under the experimental condition used.
