Figure 4.

Time course of the mandelonitrile hydrolysis mediated by the recombinant E. coli M15/BCJ2315. The reaction mixture (10 ml) containing wet cells (100 mg) and mandelonitrile (100 mM) suspended in phosphate buffer (100 mM, pH 8.0) was incubated in a rotary shaker (30°C, 200 rpm). Samples were taken every 10 min and quenched by the addition of 10% (v/v) 2 M HCl.