Table 1.

Organisms and accession numbers of the putative nitrilases mined from the GenBank database[27-31]

Organism	GenBank Accession no.	Predicted function	Defined functionb	Identitya(%)	Reference
Bradyrhizobium japonicum USDA 110	NP_773042		nitrilase	100	[21]
Burkholderia xenovorans LB400	YP_559838		nitrilase	60	[22]
Pseudomonas fluorescens EBC191	AAW79573		nitrilase	60	[17]
Sphingomonas wittichii RW1	YP_001261492	unnamed protein product		58	This study
Bradyrhizobium sp. ORS 278	YP_001206496	aliphatic nitrilase		58	This study
Bradyrhizobium sp. BTAi1	YP_001240698	aliphatic nitrilase		57	This study
uncultured organism	AAR97509		nitrilase	56	[20]
Burkholderia cenocepacia J2315	YP_002231697	putative nitrilase		56	This study
Methylibium petroleiphilum PM1	YP_001020190	aliphatic nitrilase		55	This study
Nocardia farcinica IFM 10152	YP_119480	nitrilase		55	This study
Stappia aggregata IAM 12614	ZP_01549810	nitrilase		53	This study
Rhodococcus rhodochrous K22	Q02068		nitrilase	51	[27]
Rhodococcus rhodochrous J1	Q03217		nitrilase	50	[23]
Acidovorax facilis 72W	ABD98457		nitrilase	49	[25]
Alcaligenes faecalis JM3	P20960		nitrilase	47	[13]
Sphingomonas wittichii RW1	YP_001264656	unnamed protein product		46	This study
Neosartorya fischeri NRRL 181	XP_001261815	nitrilase, putative		45	This study
Aspergillus clavatus NRRL 1	XP_001276027	nitrilase, putative		41	This study
Aspergillus niger	ABX75546		nitrilase	38	[28]
Bordetella bronchiseptica RB50	NP_887662	unnamed protein product		37	This study
Arthrobacter aurescens TC1	YP_946154	nitrilase		36	This study
Bacillus pumilus ATCC 7061	ZP_03055417	nitrilase		36	This study
Rhodopseudomonas palustris CGA009	NP_949502	nitrilase		36	This study
Pseudomonas fluorescens Pf-5	YP_260565	carbon-nitrogen family hydrolase		36	This study
Arabidopsis thaliana	NP_190018		nitrilase	35	[29]
Burkholderia graminis C4D1M	ZP_02885032	nitrilase		35	This study
Bradyrhizobium japonicum USDA 110	NP_770037		nitrilase	35	[30]
Arabidopsis thaliana	NP_197622		nitrilase	35	[31]
Methylibium petroleiphilum PM1	YP_001022666	nitrilase		34	This study
Synechocystis sp. PCC 6803	NP_442646		nitrilase	34	[24]
Burkholderia phymatum STM815	YP_001861308	unnamed protein product		34	This study
Arabidopsis thaliana	NP_190016		nitrilase	34	[29]
Arabidopsis thaliana	NP_851011		nitrilase	34	[29]
Shewanella pealeana ATCC 700345	YP_001502716	nitrilase		33	This study
Burkholderia xenovorans LB400	YP_559598	nitrilase		32	This study
Roseobacter sp. GAI101	ZP_05099070	aliphatic nitrilase		32	This study
Burkholderia ambifaria AMMD	YP_772488	nitrilase/cyanide hydratase hydratase and apolipoprotein N-acyltransferase		31	This study
Pseudomonas fluorescens Pf-5	YP_260015		nitrilase	31	[26]
Zymomonas mobilis ZM4	YP_162942	unnamed protein product		30	This study

^a BLASTP was performed by comparing the amino acid sequence with that of the mandelonitrile hydrolase (bll6042, NP_773042) from Bradyrhizobium japonicum USDA 110.

^b The enzymes were experimentally characterized to harbor nitrilase activity.