Table 3.
Substrate specificity of the purified BCJ2315
| Entry | Substrate | Relative activitya(%) |
|---|---|---|
| 1 |
Iminodiacetonitrile |
21±1 |
| 2 |
acrylonitrile |
17±1 |
| 3 |
2-Methylglutaronitrile |
25±3 |
| 4 |
Succinonitrile |
5±1 |
| 5 |
Fumaronitrile |
78±3 |
| 6 |
Malononitrile |
17±1 |
| 7 |
Valeronitrile |
4±1 |
| 8 |
3-Hydroxyglutaronitrile |
N.D. |
| 9 |
4-Chlorobutyronitrile |
11±1 |
| 10 |
Glycolonitrile |
69±4 |
| 11 |
3-Phenylpropionitrile |
N.D. |
| 12 |
Benzonitrile |
N.D. |
| 13 |
Cinnamonitrile |
N.D. |
| 14 |
Phenylacetonitrile |
100±4 |
| 15 |
2-Chloromandelonitrile |
298±7 |
| 16 |
1,2-Phenylenediacetonitrile |
37±5 |
| 17 |
2-Phenylbutyronitrile |
N.D. |
| 18 |
alpha-Methylphenylacetonitrile |
N.D. |
| 19 |
mandelonitrile |
884±7 |
| 20 |
3-Cyanopyridine |
N.D. |
| 21 |
Thiophene-3-carbonitrile |
1±1 |
| 22 |
Indole-3-acetonitrile |
20±1 |
| 23 |
2-Cyanopyridine |
7±1 |
| 24 | 4-Cyanopyridine | 7±1 |
a Nitrilase activity against various nitriles was measured under standard assay conditions. The activity toward phenylacetonitrile was defined as 100%.
N.D. not detected.