Table 4.
Kinetic parameters, specific activity, enantioselectivity, and specificity of BCJ2315 compared with other mandelonitrile hydrolases[33-35]
| Organism |
Kinetic parameters |
Specific activity (U/mg) | ee value of acid (%) | Amide formation (% of total products) | Reference | |
|---|---|---|---|---|---|---|
| Km(mM) | Vmax(μmol/min/mg) | |||||
|
uncultured organisma |
N.A. |
N.A. |
50 |
98 |
N.D. |
[34] |
|
Pseudomonas fluorescens EBC191 |
N.A. |
N.A. |
32.8 |
31 |
19 |
[19] |
|
Burkholderia cenocepacia J2315 |
0.14 |
45.4 |
27.79 |
98.4 |
N.D. |
This study |
|
Bradyrhizobium japonicum USDA110b |
0.26 |
44.7 |
24.38 |
- |
N.D. |
[21] |
|
Alcaligenes sp. ECU0401 |
21.8 |
27.9 |
19 |
97 |
N.D. |
[11] |
|
Alcaligenes faecalis JM3 |
N.A. |
N.A. |
12.4 |
99 |
N.D. |
[13] |
|
Aspergillus niger CBS 513.88 |
11.4 |
12.4 |
7.7 |
99 |
N.D. |
[16], [35] |
|
Neurospora crassa OR74A |
3.4 |
9.9 |
6.1 |
99 |
15 |
[16], [35] |
|
Pseudomonas putida |
13.39 |
16.5 |
3.26 |
99.9 |
N.D. |
[33] |
|
Alcaligenes faecalis ATCC8750 |
N.A. |
N.A. |
3.1 |
100 |
N.D. |
[10] |
|
Burkholderia xenovorans LB400 |
0.084 |
N.A. |
1.523 |
- |
N.D. |
[22] |
| Alcaligenes faecalis ZJUTB10 | 4.74 | 15.85 | N.A. | 99 | N.D. | [12] |
N.A. data not available.
N.D. not detected.
- no enantioselectivity was observed.
a nitrilase I (2A6) was referred here.
b nitrilase (bll6402) was referred here.