. 2013 Feb 18;13:14. doi: 10.1186/1472-6750-13-14

Table 5.

Primers and locus tags of the nitrilase genes in the predicted mandelonitrile hydrolase subgroup used in this study

Sequence Accession no.	Locus_tag	Source	Primer pair (sequence 5’→ 3’)	Restriction enzymes
CP000699	Swit_0987	Sphingomonas wittichii RW1^a	Forward: CATATGTCGGACGGTCCGTTCAAGGTTG	NdeI/HindIII
CP000699	Swit_0987	Sphingomonas wittichii RW1^a	Reverse: AAGCTTTTAGGAACCGGCCTTTTCGGCGA	NdeI/HindIII
CU234118	BRADO4539	Bradyrhizobium sp. ORS 278^a	Forward: GGGAATTCCATATGGGACTGGCACATCCG	NdeI/HindIII
CU234118	BRADO4539	Bradyrhizobium sp. ORS 278^a	Reverse: GAAAAAAGCTTACCCGCCAGCCGCGACCT	NdeI/HindIII
CP000494	BBta_4766	Bradyrhizobium sp. BTAi1^b	Forward: GGATCCATGGGACTGGCACATCCGAAATAC	BamHI/HindIII
CP000494	BBta_4766	Bradyrhizobium sp. BTAi1^b	Reverse: AAGCTTCAGATGGATTGATCGGGCCGCGCG	BamHI/HindIII
AM747720	BCAL2585	Burkholderia cenocepacia J2315^b	Forward: GGATCCATGACCATCAATCACCC	BamHI/HindIII
AM747720	BCAL2585	Burkholderia cenocepacia J2315^b	Reverse: AAGCTTAAGCGGGTGTGACGC	BamHI/HindIII
CP000555	Mpe_A0993	Methylibium petroleiphilum PM1^a	Forward: CATATGCCGGTTTCGCACCCCAAG	NdeI/HindIII
CP000555	Mpe_A0993	Methylibium petroleiphilum PM1^a	Reverse: AAAAAAGCTTAAGCCGTGCGGCGCGCGGTC	NdeI/HindIII
BAD58116	nfa32690	Nocardia farcinica IFM 10152^a	Forward: CATATGAGTCAGCGAGACAGTTTCCG	NdeI/HindIII
BAD58116	nfa32690	Nocardia farcinica IFM 10152^a	Reverse: ATATAAGCTTCCGCACCGCGGGTTCGGCGT	NdeI/HindIII
NZ_AAUW01000019	SIAM614_26356	Stappia aggregata IAM 12614^a	Forward: CATATGAAAGCTATCAAGGTTGCCGCCGTTC	NdeI/BamHI
NZ_AAUW01000019	SIAM614_26356	Stappia aggregata IAM 12614^a	Reverse: GGATCCCTACTCCTCGACCTCAAAAGGCCGT	NdeI/BamHI

^a The gene was cloned into a pET28a(+) expression vector using E. coli BL21 (DE3) as host strain.

^b The gene was cloned into a pQE30 expression vector using E. coli M15 as host strain.