Table 1.
Primers used for RT-qPCR in the present study
| |
|
|
|
Primer efficiency |
|
|
|
|---|---|---|---|---|---|---|---|
| Target gene | Forward primer (5′- 3′) | Reverse primer (5′- 3′) | Amplicon size | Fn. ssp. noatunensis | Fn. ssp. orientalis | Gene ID | Position |
|
uvrD |
ACTATTTGTCGCGGGTCCTT |
TCAAAGAAACGAAAACCTCCA |
82 bp |
2.050 |
1.959 |
12951493 |
596634-596715 |
|
rpoB |
GTGGTAAAGCGCAATTTGGT |
CAGCACCATATGCTTGTAACG |
72 bp |
1.986 |
1.988 |
12951517 |
620011-620082 |
|
gyrA |
CGAGCTTTACGAGCTGCTTC |
TCTTTTAGAGAACCCTAAAGAGGCT |
87 bp |
1.982 |
2.000 |
12952071 |
1187616-1187702 |
|
polA |
AGCTGGAACTGGTCGTAATCA |
ATCAGCATCTTCAGCAGCATA |
82 bp |
1.959 |
1.950 |
12951484 |
584274-584355 |
|
fopA |
TACTGGTGCATGGGATGTTG |
TCTTGGAGCCATTGTCTGAA |
100 bp |
1.902 |
1.938 |
12952182 |
1297252-1297351 |
|
ftsZ |
TACCATACTCAGCGGCTTTC |
GCGCCTGTAGTTGCTGAAGT |
112 bp |
1.986 |
1.997 |
12952792 |
136099-136210 |
|
ribC |
ATCTCAACTAGCCACGCTCC |
CGGTGGACACATGGTACAAG |
87 bp |
1.946 |
1.950 |
12952738 |
84136-84222 |
|
16S rRNA |
AACGACTGTTAATACCGCATAATATCTG [17] |
CCTTACCCTACCAACTAGCTAATCCA [17] |
101 bp |
1.954 |
1.954 |
12951375 |
463870-463970 |
| iglC | TAGGCGTATAACACTGGCTGC | TGCTATAGAAGGCGGAGAGG | 70 bp | 2.006 | 1.905 | 12951826 | 930145-930214 |
The complete genome of F. noatunensis ssp. orientalis strain Toba 04 (Accession number NC_017909), was used as a reference to assign the Gene ID.