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. 2013 Feb 26;13:31. doi: 10.1186/1471-2229-13-31

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Copyright ©2013 Boubakri et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time course of transcript accumulation of genes of the phenylpropanoid pathway after thiamine treatment. Grapevine plants (cv. Chardonnay) were treated with 30 mM thiamine (solid bars) or water (white bars) and leaves were collected at different time intervals post-treatment (12, 24, 36, 48, and 72 hpt). Gene expression was analyzed by real-time q-PCR. Genes of interest are: (A) PAL (phenylalanine ammonialyase), (B) C4H1 (cinnamate 4-hydroxylase), (C) 4CL1 (4-coumarate:coenzyme A ligase), (D) CCR2 (cinnamoyl:coenzyme A reductase), (E) CHS1 (chalcone synthase) and (F) STS1 (stilbene synthase1). Results are presented as normalized fold-expression of defense genes in thiamine-treated samples compared to expression in water-treated controls at the same time point, which is set to 1. Results are mean ± standard deviations of 3 independent experiments.